1Laboratory of Physics of Living Matter, EPFL, Lausanne, Switzerland
2School of Natural Sciences and Engineering, Ilia State University, Tbilisi, Georgia
3Institute for multidisciplinary research, University of Belgrade, Belgrade, Serbia
4Plateforme de Morphologie, Université de Lausanne, Switzerland
Received: 31 Oct 2016 – Revised: 26 Jan 2017 – Accepted: 28 Feb 2017 – Published: 14 Mar 2017
Abstract. Our team recently demonstrated that cantilever based devices can detect signature of life in a chemistry independent manner. In this technique, the organism of interest is attached onto a classical AFM cantilever. If alive, it induces nanometre scale oscillations of the cantilever that disappear once the organism is killed. The technique was successfully used on bacteria, yeast, vegetal and mammalian cells. In this work we demonstrate that the method can also be applied to sub-cellular organelles, such as mitochondria. Mitochondria are involved in cellular energy production and are present in most eukaryotic cells. Nowadays, it is believed that mitochondria were originally prokaryotes that colonized eukaryotic cells and that live in an endosymbiotic way ever since. Here we present that mitochondria are also animated by nanometre scale oscillations that depend on their metabolic state and that stop once they are inhibited. This observation opens novel avenues to investigate the numerous mitochondria-related diseases in humans.
Stupar, P., Chomicki, W., Maillard, C., Mikeladze, D., Kalauzi, A., Radotić, K., Dietler, G., and Kasas, S.: Mitochondrial activity detected by cantilever based sensor, Mech. Sci., 8, 23-28, doi:10.5194/ms-8-23-2017, 2017.